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. 2016 Sep 1;16(1):65. doi: 10.1186/s12896-016-0294-5

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — The apoptosis of tumor cells induced by GBP-SubA. a. HL-7702, DLD1 and HepG2 cells were incubated with 0.1 μg/mL GBP-SubA for 12 h. The entry of GBP-SubA to cells was analyzed by immunofluorescence. The GBP-SubA was stained by anti-His-tag primary antibody and FITC-conjugated secondary antibody, the actin was stained by Rhodamine-conjugated Phalloidin, and the nucleus was stained by DAPI. b. HL-7702, DLD1 and HepG2 cells were incubated with 0.1 μg/mL GBP-SubA for 48 h, the control was treated with PBS, and the apoptosis was analyzed by flow cytometry