Table 3.

Genes that are upregulated in the Treg population B compared with population A

Genes	FDR q-value	Normalized enrichment score	Significant genes
G2M checkpoint	<0.0001	2.0188198	CASC5, NUSAP1, CENPE, TPX2, TOP2A, KIF11, BARD1, EZH2, SLC7A5, HN1, GINS2, CKS2, BUB1, SMC4, STIL, BRCA2, CHEK1, SAP30, E2F3, MKI67, CDKN2C, NEK2, KIF15, KPNA2, KIF23, ZAK, POLQ, WHSC1, TFDP1, UCK2, E2F2, CDKN3, CDC7, E2F1, AURKA, CDC20, EFNA5, KIF4A, EXO1, CDC25A, PRC1, KIF5B
Mitosis	0.014934987	2.020	NCAPH, NUSAP1, NDC80, CENPE, BUB1B, TPX2, KIF11, TTN, BUB1, NEK2, SMC4, PCBP4, PAM
M phase of mitotic cell cycle	0.01755665	2.0006573	NCAPH, NUSAP1, NDC80, CENPE, BUB1B, TPX2, KIF11, TTN, BUB1, NEK2, SMC4, PCBP4, PAM
IL2-STAT5 signaling	0.02301426	1.5519865	SYT11, CCR4, TNFRSF9, CST7, ADAM19, IL1R2, ANXA4, PHLDA1, SLC1A5, IL18R1, FGL2, TNFRSF18, TNFRSF4, GALM, CXCL10, BATF, SPP1, TNFSF10, PHTF2, CD86, AHNAK, IL10, LIF, TLR7, F2RL2, CD79B, CTLA4, FURIN, TNFRSF1B, CAPG, ALCAM, CSF1, CASP3, CSF2, MUC1, MYO1E RORA, ITGAV, PRNP, ICOS, UCK2, CYFIP1, SOCS1
Immune response genes	0.031976275	1.9183398	IL7, CCR2, CCR8, CCR4, IL1R2, CCR6, CST7, CCL20, GZMA, CADM1, AIM2, CIITA, CCR5, CTSC, IL12A, NCF4, IL4, CCR9, GEM, IL32, TNFRSF4, LAX1, DEFB4A, FCGR3B, TLR7, CD74, APOA4, CCL5, APOBEC3G, CD79B, CTLA4

The most significant gene sets that are upregulated in the Treg B subpopulation compared with Treg A are listed. Leading-edge analysis revealed several genes that significantly contribute in each gene set and are enriched in Treg B compared with Treg A (marked as significant genes).