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. 2016 Jan 5;150(1):117–130. doi: 10.1093/toxsci/kfv314

FIG. 6.

FIG. 6.

Effects of ruthenium red (RR) pretreatment on the methylmercury (MeHg)-induced initial stimulatory effects on miniature inhibitory postsynaptic currents (mIPSCs). A–B, mIPSCs were recorded from a Purkinje cell in a slice at a holding potential of −60 mV in the presence of 10 µM 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), 50 µM amino-5-phosphonopentanoic acid (APV) and 1.0 µM TTX. The slice was first incubated with 20 μM RR in regular artificial cerebrospinal fluid (ACSF) for 15 min (A) and then exposed to 100 μM MeHg plus 20 μM RR (MeHg+RR) in regular ACSF for 6 min (B). C, Effect of MeHg plus RR on cumulative fraction of inter-event intervals of mIPSCs from the same cell shown in (A). Bin size: 100 ms. D, Effect of MeHg on amplitude distribution of mIPSCs from the same cell in (A). Bin size: 10 pA. E–F, Comparisons of MeHg-induced changes in relative frequency or amplitude of mIPSCs in in the presence of RR in regular ACSF. Each trace is a representative depiction of 5 individual experiments. The asterisk indicates a statistically significant difference between the 2 groups (P < .05).