FIG. 8.

Comparison of effects of methylmercury (MeHg) on fluo-4 fluorescence intensity in the ML of cerebellar slices with or without pretreatment with low [Ca²⁺]_o, caffeine or cyclopiazonic acid (CPA). Slices were treated with MeHg alone, or in combination with application of low [Ca²⁺]_o, 10 mM caffeine or 25 μM CPA, respectively. Data were normalized to that of prior to MeHg treatment (“Control”). Note the labels: the control actually represents pretreatment with low Ca²⁺, caffeine, or CPA; MeHg, MeHg treatment alone in artificial cerebrospinal fluid (ACSF), MeHg+Caff, MeHg plus caffeine treatment; MeHg+CPA, MeHg plus CPA treatment; and MeHg+Low Ca²⁺, MeHg in low Ca²⁺-containing ACSF. The asterisks indicate significant differences between control and MeHg treatment in the absence or presence of caffeine, CPA or low [Ca²⁺]_o (P < 0.05, Student’s paired t test). The values are mean ± SEM of 4–7 individual experiments