FIG. 3.

Effect of acute di(2-ethylhexyl) phthalate (DEHP) exposure on serum gonadotropin and peptide hormone levels at 9 months postdosing. Adult CD-1 mice were orally dosed with vehicle (tocopherol-stripped corn oil) or DEHP (20 µg/kg/day-500 mg/kg/day) daily for 10 days. Following 9 months postdosing, serum was collected, sent to the University of Virginia Center for Research in Reproduction Ligand Assay and Analysis Core, and subjected to enzyme-linked immunosorbent assays (ELISAs) or radioimmunoassays (RIAs) for the measurements of follicle-stimulating hormone (FSH) (panel A), luteinizing hormone (LH) (panel B), inhibin B (panel D), and AMH (panel E). Additionally, the FSH/LH ratio was calculated (panel C). Graphs represent means ± SEM (n = 3–6 mice/treatment group). Asterisks (*) represent significant difference from vehicle control (P ≤ 0.05).