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Standardized protocol for precision cut lung slice (PCLS) preparation. A, The agarose-infused left lung lobe was removed. B, The left lung lobe was then trimmed. C, A slab of lung tissue was sliced using a vibratome. Consecutive lung slices were generated from the trimmed slab. D, Sequential slices were assigned alternately to each experimental condition as shown. Complementary PCLS were cryopreserved in cryovials prior to experimentation.
