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. Author manuscript; available in PMC: 2016 Sep 2.
Published in final edited form as: Neuropharmacology. 2012 May 2;63(3):427–433. doi: 10.1016/j.neuropharm.2012.04.028

Fig. 3.

Fig. 3

Receptor binding characteristics of [3H]N/OFQ to spinal cord membranes of male and female rats. Membrane preparations (n = 6 in each group) were incubated with 0.9–6 nM [3H]N/OFQ; specific binding was determined and results subjected to Scatchard analysis as described in Methods. A, B. Scatchard transformations of representative binding data. C. NOP receptor levels increased in SC membranes from morphine-treated rats (**p < 0.001) compared to saline-treated rats; no significant effect of sex was noted. D. NOP receptor affinity in spinal cord membranes decreased after chronic morphine treatment (**p < 0.001), with no significant effect of sex. Data were analyzed by two-way ANOVA.