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. 2016 Sep 1;9(1):430. doi: 10.1186/s13104-016-2224-y

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — RNA transcript levels of six target genes from adult and cord blood samples. RNA isolated from adult and cord blood samples using five different protocols and analyzed by qPCR. a Relative transcript levels of six genes from adult blood samples collected in Tempus tubes (n = 11–12 Tempus tubes for each protocol). b Relative transcript levels of six genes from cord blood samples collected in Tempus tubes (n = 3–6 Tempus tubes for each protocol). The Tempus 6-port samples were used as reference samples (calibrators) and all other samples were compared against the reference samples. Each bar represents the average log2-transformed fold change values; fold change = 2^−∆∆Cq. The error bars indicate ± SE and the stippled lines indicate ± twofold