Fig. 3.

Non-normalized raw Cq-value and coefficients of variation of 13 miRNAs. RNA isolated from adult and cord blood using three different RNA isolation protocols optimized for simultaneous isolation of all RNA species and analyzed by qPCR. The non-normalized raw Cq-values for adult blood (n = 12 Tempus tubes) and cord blood (n = 3 Tempus tubes) samples collected in the Tempus tubes. a Average non-normalized raw Cq-values for adult blood samples; b the average non-normalized raw Cq-values for cord blood samples. The error bars indicate ± SE. There are no significant differences in the raw Cq-values between the three RNA isolation protocols. The coefficients of variation (CV) of the average raw Cq-values were calculated for adult and cord blood samples for each RNA isolation protocol for the 13 miRNAs. c CVs for adult blood samples (ranging from 4.5 to 18.8 %); d CVs for cord blood samples (ranging from 1.7 to 10.9 %). CV of 10 and 20 % is indicated by stippled lines. Each point represents the average CV of samples for one miRNA from one Tempus tubes from one protocol