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. 2016 Sep 1;9(1):430. doi: 10.1186/s13104-016-2224-y

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Log2-NRQ values for 13 miRNAs for cord blood samples. The normalized relative quantity values [NRQ = 2^−ΔCq(sample); where the ΔCq (sample) = Cq (miRNA) − Cq (internal control)]) of 13 miRNAs from cord samples collected in the Tempus tubes (n = 3 Tempus tubes for adult blood). a Log2-transformed NRQ-value of 13 miRNAs for the three RNA isolation protocols. Each bar represents the log2-transformed NRQ values and the error bar indicates ± SE; b correlation between MagMax semi-automated and manual protocols; c correlation between MagMax semi-automated and Norgen protocols; d correlation between MagMax manual and Norgen protocols. There are significant (p < 0.001) correlations between the NRQ-values of the three protocols. Each point represents the average of log2-NRQ values of three technical replicates from one Tempus tube, i.e., 13 miRNAs × 3 blood samples from 3 Tempus tubes