Table 2.
Comparison of RNA QC using five RNA isolation protocols
| RNA isolation protocol | RIN value | OD 260/280 ratio | OD 260/230 ratio | Number of Tempus tubes |
|---|---|---|---|---|
| (a) Adult blood | ||||
| MagMAX semi-automated | 7.85 ± 0.13 | 2.09 ± 0.01 | 1.97 ± 0.05 | 12 |
| MagMAX manual | 7.20 ± 0.11 | 2.12 ± 0.01 | 1.19 ± 0.12* | 12 |
| Norgen | 7.36 ± 0.12 | 2.12 ± 0.02 | 1.76 ± 0.07* | 12 |
| Tempus spin | 8.97 ± 0.05 | 2.09 ± 0.01 | 2.08 ± 0.01 | 12 |
| Tempus 6-port | 8.38 ± 0.08 | 2.20 ± 0.06 | 2.02 ± 0.05 | 11 |
| Average | 7.93 ± 0.10 | 2.12 ± 0.01 | 1.80 ± 0.05 | 59 |
| (b) Cord blood | ||||
| MagMAX semi-automated | 7.47 ± 0.22 | 2.09 ± 0.03 | 2.01 ± 0.09 | 3 |
| MagMAX manual | 7.73 ± 0.47 | 2.12 ± 0.01 | 2.14 ± 0.02 | 3 |
| Norgen | 8.23 ± 0.33 | 2.11 ± 0.01 | 2.07 ± 0.04 | 3 |
| Tempus spin | 7.13 ± 0.13 | 2.10 ± 0.01 | 2.11 ± 0.01 | 3 |
| Tempus 6-port | 8.37 ± 0.38 | 2.10 ± 0.01 | 2.10 ± 0.02 | 6 |
| Average | 7.88 ± 0.19 | 2.10 ± 0.02 | 2.09 ± 0.05 | 18 |
* RNA extracted with MagMAX manual and Norgen protocols have significantly lower OD 260/230 ratio compared to RNA from the other protocols, p < 0.05