Table 3.
Raw Cq-value and CVs (coefficients of variation) for adult blood samples
| RNA isolation protocol | CDKN1A | FOS | IL1B | IL8 | MYC | TP53 |
|---|---|---|---|---|---|---|
| MagMax semiautomated | ||||||
| Average | 30.5 | 26.9 | 28.4 | 30.4 | 29.1 | 28.3 |
| SD | 0.7 | 0.2 | 0.8 | 0.3 | 0.4 | 0.4 |
| % CV | 2.1 | 0.8 | 3.0 | 1.1 | 1.3 | 1.3 |
| MagMax manual | ||||||
| Average | 30.1 | 26.5 | 27.9 | 30.0 | 28.8 | 27.8 |
| SD | 0.7 | 0.3 | 0.7 | 0.3 | 0.4 | 0.3 |
| % CV | 2.5 | 1.2 | 2.6 | 1.1 | 1.2 | 1.0 |
| Norgen | ||||||
| Average | 30.7 | 26.6 | 28.2 | 30.2 | 29.9 | 28.7 |
| SD | 0.7 | 0.4 | 0.8 | 0.4 | 0.4 | 0.4 |
| % CV | 2.3 | 1.4 | 2.7 | 1.2 | 1.3 | 1.3 |
| Tempus spin | ||||||
| Average | 29.4 | 26.1 | 27.9 | 29.5 | 28.9 | 27.8 |
| SD | 0.7 | 0.3 | 0.8 | 0.4 | 0.3 | 0.4 |
| % CV | 2.4 | 1.1 | 2.8 | 1.3 | 1.2 | 1.4 |
| Tempus 6-port | ||||||
| Average | 29.9 | 26.4 | 28.0 | 29.9 | 29.1 | 28.0 |
| SD | 0.8 | 0.2 | 0.7 | 0.3 | 0.3 | 0.3 |
| % CV | 2.5 | 0.8 | 2.4 | 1.0 | 1.1 | 1.0 |
| Overall average | ||||||
| Average | 30.1 | 26.5 | 28.1 | 30.0 | 29.2 | 28.1 |
| SD | 0.9 | 0.4 | 0.8 | 0.5 | 0.5 | 0.5 |
| % CV | 2.8 | 1.5 | 2.8 | 1.5 | 1.8 | 1.7 |
CVs are shown in italics; the overall averaged CVs range between 1.5 and 2.8 %