Table 4.
Raw Cq-value and CVs (coefficients of variation) for cord blood samples
| RNA isolation protocol | CDKN1A | FOS | IL1B | IL8 | MYC | TP53 |
|---|---|---|---|---|---|---|
| MagMax semiautomated | ||||||
| Average | 32.5 | 28.0 | 29.8 | 32.2 | 29.9 | 29.5 |
| SD | 0.5 | 0.9 | 0.6 | 1.2 | 0.3 | 0.2 |
| % CV | 1.4 | 3.2 | 2.0 | 3.8 | 1.1 | 0.7 |
| MagMax manual | ||||||
| Average | 32.0 | 28.2 | 29.5 | 32.6 | 29.4 | 29.1 |
| SD | 0.2 | 0.3 | 0.3 | 0.4 | 0.2 | 0.2 |
| % CV | 0.6 | 1.1 | 1.1 | 1.3 | 0.7 | 0.6 |
| Norgen | ||||||
| Average | 32.7 | 28.2 | 29.7 | 31.5 | 30.0 | 29.6 |
| SD | 0.6 | 1.4 | 1.0 | 2.4 | 0.3 | 0.5 |
| % CV | 1.8 | 5.1 | 3.5 | 7.5 | 1.1 | 1.6 |
| Tempus spin | ||||||
| Average | 31.8 | 28.0 | 28.8 | 31.2 | 29.3 | 28.7 |
| SD | 0.2 | 0.3 | 0.2 | 0.4 | 0.1 | 0.2 |
| % CV | 0.7 | 1.2 | 0.7 | 1.4 | 0.4 | 0.7 |
| Tempus 6-port | ||||||
| Average | 33.0 | 29.0 | 30.1 | 32.8 | 31.0 | 29.9 |
| SD | 0.6 | 0.5 | 0.3 | 0.4 | 0.8 | 0.4 |
| % CV | 1.8 | 1.8 | 0.9 | 1.3 | 2.5 | 1.2 |
| Overall average | ||||||
| Average | 32.5 | 28.4 | 29.7 | 32.2 | 30.1 | 29.5 |
| SD | 0.6 | 0.9 | 0.7 | 1.3 | 0.8 | 0.5 |
| % CV | 2.0 | 3.0 | 2.3 | 3.9 | 2.8 | 1.8 |
CVs are shown in italics; the overall averaged CVs range between 1.8 and 3.9 %. The average CV value of the IL8 gene isolated with the Norgen protocol had the highest CV value (CV = 7.5 %) and shown in bolditalics