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. 2016 Sep 1;16(1):176. doi: 10.1186/s12862-016-0747-4

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Measurement of tilting index parameters. Tilting index TI_i (t) at a certain time point t in mating couple i was calculated by measuring the following parameters: a distance between the bases of the wings in the male during copulation d(M _i ,t), b distance between the bases of the wings in the female during copulation d(F _i ,t), c distance between the bases of the wings in the male in resting position d(M _i , restingposition) and d distance between the bases of the wings in the female in resting position d(F _i , restingposition). Note that measurements of flies in resting position were taken when they were positioned upside-down on the Plexiglas cover of the mating cell, to be certain that the flies were positioned on a horizontal surface