Table 2.
Mating behaviour and mating position in wild-type D. pachea couples with unmodified males, ‘left lobe cut’ males and ‘left lobe shaved’ males
| Unmodified wild-type | Left lobe cut | Left lobe shaved | Wild-type in Lang & Orgogozo 2012 [24] | Symmetric mutant in Lang & Orgogozo 2012 [24] | |
|---|---|---|---|---|---|
| Total number of couples | 16 | 18 | 18 | 19 | 22 |
| Number of couples that failed to copulate | 0 | 1 | 0 | 0 | 8 |
| Number of males that did copulate, but failed to do so at first attempt | 0 | 2 | 2 | 0 | 3 |
| Time until male courtship started [min:sec; mean ± SD] | 9:53 ± 13:12 (n = 16) | 5:58 ± 9:41 (n = 17) | 9:06 ± 14:35 (n = 18) | 10:56 ± 26:39 (n = 19) | 1:52 ± 1:45 (n = 22) |
| Duration of male courtship until copulation occurred [min:sec; mean ± SD] | 9:38 ± 8:15 (n = 16) | 20:18 ± 25:07 (n = 16) | 12:13 ± 32:03 (n = 18) | 6:15 ± 7:15 (n = 19) | 13:30 ± 20:04 (n = 14) |
| Duration of copulation until female started kicking [min:sec; mean ± SD] | 17:11 ± 8:00 (n = 14) | 14:21 ± 4:17 (n = 14) | 13:28 ± 3:58 (n = 14) | not recorded | not recorded |
| Total duration of copulation [min:sec; mean ± SD] | 27:38 ± 6:46 (n = 16) | 27:35 ± 6:22 (n = 17) | 25:28 ± 5:20 (n = 18) | 34:55 ± 7:08 (n = 19) | 39:55 ± 10:56 (n = 14) |
| Mating angle at 10–15 min after copulation starts (present study) or at 4–11 min [24] [degree; mean ± SD] | 6.06 ± 3.64 (n = 12) | −1.98 ± 11.13 (n = 12) | 8.87 ± 11.70 (n = 14) | 8.55 ± 1.79 (n = 12) | 5.77 ± 5.33 (n = 11) |
| Tilting index at 10–15 min after copulation starts [mean ± SD] | 0.960 ± 0.038 (n = 12) | 0.880 ± 0.070 (n = 12) | 0.909 ± 0.096 (n = 14) | not recorded | not recorded |
| Male mortality between date of treatment and date of video recording or snap-freezing (including males used for snap-freezing experiments) | 26.9 % (n = 14/52) | 18.0 % (n = 9/50) | 17.1 % (n = 6/35) |
Data from a previous study [43] are incorporated. Note that courtship time is higher in left lobe cut males than in control males but that this difference is not significant (log-transformed; LM: F 2,47 = 1.70, P = 0.194). Courtship duration is the time interval between the moment of first apparent male courtship behaviour and the moment at which the male mounts the female. SD = standard deviation, n = number of observations