Table 2. Template usage under ensemble conditions is comparable to template usage from complexes formed on surface-immobilized DNA.
| Green spots | Red spots | Colocalized spot pairs | % of green spots that have a colocalized red | |
|---|---|---|---|---|
| Ensemble transcription | 3932 | 1086 | 479 | 12.2 |
| Transcription on surface (-NTPs) | 5561 | 901 | 172 | 3.1 |
| Transcription on surface (+NTPs) | 5523 | 2713 | 682 | 12.4 |