Skip to main content
. 2016 Apr 25;44(15):7144–7158. doi: 10.1093/nar/gkw323

Figure 6.

Figure 6.

Loss of HMGNs enhances the occupancy of unstable nucleosomes at TSS in both resting and activated B cells. (A and B) Loss of HMGNs does not affect the kinetics of MNase digestion of resting cells. (C and D) Loss of HMGNs enhances the occupancy of unstable (C) but not of stable (D) nucleosomes at the TSS of resting cells. (E and F) Loss of HMGNs does not affect the kinetics of MNase digestion of activated cells. (G and H) Loss of HMGNs enhances occupancy of unstable (G) but not of stable (H) nucleosome at the TSS of activated cells. Resting and activated B cells were digested with increasing concentration of MNase (0.01–2 U/ml). Mononucleosomes from limited (L) and extensive (E) digestions were isolated, their DNA sequenced and aligned to the TSS of top 3000 expressed genes. Arrow points to the ‘nucleosome free’ region near the TSS.