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. 2016 Apr 25;44(15):7159–7172. doi: 10.1093/nar/gkw324

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — Gcn5 promotes histone H3 acetylation and H3 eviction at MLP1 but not at YLR194C gene. (A) H3 acetylation was determined by ChIP analysis in a WT strain treated with CR for the indicated time points at MLP1-BOX1 region, using antibodies specific for diacetylated H3 (K9 and K14) (acH3) and against total H3 (H3). Values are expressed as the ratio of acetylated H3 versus total H3 enrichment. (B) H3 acetylation (left panel) and H3 eviction (right panel) was determined at MLP1-BOX1 region in WT and gcn5Δ strains after 3 h of CR addition. (C) mRNA levels of MLP1 were analyzed by RT-qPCR in YJS6 (GCN5), YKH100 (gcn5Δ) and YJS7 (gcn5^hat) strains after 3 h of CR addition. Values represent the ratio between CR-treated and non-treated cells. (D) H3 acetylation (left panel) and H3 enrichment (right panel) were analyzed in YLR194C-BOX region as in (B). Data represent the mean and standard deviation of at least three independent experiments.