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Loss of histone chaperones perturbs nascent chromatin organization. Normalized frequency of nascent nucleosomal dyads aligned to the TSS of all genes (n = 5015) in wild-type (orange), cac1Δ (blue) (A) and asf1Δ (blue) (C) deficient strains, 10 min following addition of EdU to an asynchronously growing culture. The normalized frequency of input chromatin prior to enrichment for newly replicated fragments for wild-type, cac1Δ (B) and asf1Δ strains (D).
