Figure 6.

Alteration of nucleosome spacing in nascent chromatin in the absence of Cac1. Isolation of nascent DNA by isotope labelling and caesium chloride gradient density separation provides a means to track the relatively slow maturation of chromatin in a cac1Δ mutant. Normalized frequency of nucleosomal dyads aligned to the TSS for replicated HL (blue) and unreplciated HH (orange) labelled nucleosomal fragments isolated 33 (A), 38 (B), 43 (C), 48 (D), 55 (E), 60 (F) and 80 min (G) following release from G1 arrest. Quantitation of the 3′ shift in the average nucleosome location for +1, +2, +3 and +4 nucleosomes for each time point is shown in (H).