Figure 5.

Testing for the functional interaction between DNA fragments containing (A) binding sites for Pita and ZIPIC or (B) binding sites for Pita and Zw5 (gray circles) in the GAL4/white model system. At the top is a reductive scheme of transgenic construct used to examine the functional interaction between the binding sites for the test proteins. In some transgenic lines, weak activation of the white reporter by GAL4 was observed. However, deletion of the binding sites inserted between lox sites in transgenic lines did not abolish GAL4-mediated activation, suggesting the absence of interaction between binding sites for different proteins. Other designations are as in Figure 4.