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. 2016 May 16;44(15):7242–7250. doi: 10.1093/nar/gkw439

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Expression of POLD1^exo− changes the mutation spectrum of TLS past CPD. (A) A CPD placed opposite GpC mismatch was randomly integrated into the genome using the PiggyBlock vector. TLS across the CPD results in a dual peak in the resulting cellular clone (left), while template switching results in a homogenous GC read (right). (B) The pattern of nucleotide incorporation opposite the CPD site. The percentage of each nucleotide incorporated at each position is indicated by the size of the letter of the nucleotide in the column. The total numbers of TLS events are shown. (C) A schematic representation of the opposed arrangement of CPD photoproducts in the piggyBlock plasmid (piggyBlock-op) and possible outcomes of DNA replication, only by TLS, over the lesion. (D) The pattern of nucleotide incorporation opposite the CPD in polη/polζ/xpa and polη/polζ/xpa/pold1^exo− cells in piggyBlock-op plasmid.