Figure 2.

‘Off-sized’ miRNAs and siRNAs have high rates of 3′ mismatches in Arabidopsis. (A) Mismatch rates for reference-aligned Arabidopsis sRNA-seq reads of the indicated clusters and sizes. 21: siRNA loci dominated by 21 nt RNAs. 24: siRNA loci dominated by 24 nt siRNAs (presumed het-siRNA loci). N: loci where less than 80% of reads were between 20–24 nt, and are thus presumed to not be miRNA or siRNA loci. miRNA-miRBase: High-confidence MIRNA loci from miRBase version 21. miRNA-de novo: MIRNA loci annotated by automated software. Note that most de novo annotated MIRNA loci overlapped prior miRBase annotations. Arabidopsis libraries that were analyzed are listed in Supplementary Table S1. (B) As in A, except showing normalized accumulation levels of small RNAs.