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. 2016 May 24;44(15):7267–7280. doi: 10.1093/nar/gkw473

Figure 5.

Figure 5.

Effect of 8-oxoG on expression controlled by the artificial GR-TK promoter. (A) Elements of the glucocorticoid-regulated EGFP expression vector: minimal TK promoter with transcription start (grey box with broken arrow); EGFP coding sequence (open arrow); two Nt.Bpu10I nicking sites in the NTS, used for the insertion of synthetic oligonucleotides; and glucocorticoid receptor synthetic response element (GRE). (B) Expression of vector constructs containing ‘G’ and ‘8-oxoG’ (overlaid) at the uninduced (- DEX) and induced (+ DEX) conditions. Representative fluorescence distribution plots of cells transfected with the indicated constructs and the median fluorescence values (bar graphs below). (C) EGFP expression under the conditions of co-transfectional (0 h) and delayed (8 h) induction. Representative fluorescence distribution plots and mean EGFP expression, relative to the ‘G’ construct (n = 3, ±SD).