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Comparison of standard EXPAR and biotin-based EXPAR. Real-time fluorescence curves of EXPAR on biotin-template-2 (A) or standard template (C) in response to different amounts of let-7a at 55°C. The biotin-template-2 is a standard template labeled with a biotin at the second base from its 5′ terminus. Plots for the POI of EXPAR on the biotin template (B) or standard template (D) versus logarithmic amount of let-7a.
