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. 2016 Jun 2;44(15):e130. doi: 10.1093/nar/gkw504

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — EXPAR on toehold/biotin-template-2. (A) Real-time fluorescence curves of EXPAR on standard template, biotin-template-2, or toehold/biotin-template-2 in response to 6.02 × 10⁹ copies (10 fmol) let-7a at 55°C. The biotin-template-2 is a standard template labeled with a biotin at the second base from its 5′ terminus. The toehold/biotin-template-2 is a standard template with a biotin at the second base from its 5′ terminus and a dumbbell-shaped hairpin at its 3′ terminus. (B) Real-time fluorescence curves of EXPAR on toehold/biotin-template-2 in response to different amounts of let-7a at 55°C. (C) Plots for the POI of EXPAR on toehold/biotin-template-2 versus logarithmic amount of let-7a.