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. 2016 Jul 7;44(15):7360–7372. doi: 10.1093/nar/gkw616

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Experimentally determined and modelled kinetics and protein concentration dependence of ϕC31 integrase-mediated recombination. (A–D) The dependence of recombination on integrase and RDF concentration in the experiments (A and B) and in Model 1 (C and D). Extent of recombination after 3 h was measured experimentally (A and B) or calculated using Model 1 (C and D) for the P × B (−RDF) reaction (A and C) and the L × R (+RDF) reaction (B and D), with the indicated integrase and RDF concentrations (plasmid substrate concentration 10 nM). (E) Time-courses of P × B (−RDF) and L × R (+RDF) reactions (orange and blue respectively). Reactions were with 10 nM plasmid substrate (pPB or pLR) and 400 nM integrase, and 800 nM RDF for the L × R (+RDF) reaction. The experimental data are shown by symbols and simulated kinetics (Model 1) by lines. Data points on A, B and E are shown as mean and standard deviation from three independent replicates of the experiments.