FIGURE 3.

Raptor-deficient mice lack peripheral B cells and exhibit impaired Ab production. Splenocytes (A) and peritoneal cells (B) from Raptor-null and WT control mice were stained with fluorescent-conjugated Abs and analyzed by flow cytometry. Percentage and number of B220⁺ cells were calculated for each genotype. Data are mean ± SD (n ≥ 5 mice per genotype) pooled from three independent experiments. (C) Raptor-null (n = 5) and control (n = 5) mice were immunized with KLH Ag. Sera were collected 4 wk postimmunization and analyzed by ELISA for the indicated KLH-specific Igs. *p < 0.05.