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. 2016 Aug 12;197(6):2250–2260. doi: 10.4049/jimmunol.1600492

FIGURE 4.

FIGURE 4.

Expression of Ig H and L chains are impaired in Raptor-deficient B cells, and enforced expression of IgMHEL transgene does not restore normal development. (A) BM cells from Raptor-null and WT control mice were stained with anti-B220 and anti-IC Igμ or Igκ Abs. Rag1-null mice were used as a control for loss of μ and κ expression. Data are representative of three independent experiments (n ≥ 5 mice per genotype). (B) DNA was isolated from CD19+IgM− pro- and pre-B cells from mice of the indicated genotypes. DNA was serially diluted, and PCR was used to amplify rearranged IgH and IgL genes. The EF1a gene was used as a loading control. Data shown are representative of two independent experiments. (C and D) BM and splenocytes from WT control, Raptorfl/flMb1-Cre, IgMHEL, and Raptorfl/flMb1-Cre IgMHEL mice were stained with the indicated Abs and analyzed by flow cytometry. Shown are representative plots (n = 4 mice per genotype) from two independent experiments.