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. 2016 Aug 12;197(6):2250–2260. doi: 10.4049/jimmunol.1600492

FIGURE 6.

FIGURE 6.

Inhibiting apoptosis does not rescue B cell development of Raptor-deficient B cells. (A) BM cells and splenocytes from Raptor-null and WT control mice were stained with Annexin V and 7-AAD to assess cell death and analyzed by flow cytometry. Data are mean ± SD (n ≥ 4–6 mice per genotype) calculated from two independent experiments. (B and C) BM cells from mice of the indicated genotypes were stained with Annexin V and 7-AAD to assess cell death and analyzed by flow cytometry. Data are mean ± SD (n ≥ 5 mice per genotype) and are representative of at least four independent experiments. (D and E) BM cells from mice of the indicated genotypes were stained with fluorescent-conjugated Abs and analyzed by flow cytometry. Data are mean ± SD (n ≥ 5 mice per genotype) from at least two independent experiments. (F) BM cells from mice of the indicated genotypes were stained with anti-B220 and anti-IC Igμ or Igκ Abs. Data are representative of at least two independent experiments (n ≥ 5 mice per genotype). *p < 0.05.