Skip to main content
. Author manuscript; available in PMC: 2016 Sep 2.
Published in final edited form as: Neuropharmacology. 2014 Feb 12;87:188–197. doi: 10.1016/j.neuropharm.2014.02.002

Figure 1. Schematic representations of the experimental treatments and behavioral analysis.

Figure 1

A) Male C57BL/6 mice were subjected to sub-chronic treatment with saline or METH 1 mg/Kg, sc, once a day for 7 consecutive days. On day 4 after sub-chronic treatment, mice received vehicle or MOD 90 or 30 mg/Kg, ip, 1 hr before behavioral analysis. B) METH withdrawal mice were habituated to the object recognition arena 5 min a day for 3 consecutive days. On withdrawal day 4, mice performed the Novel Object Recognition (NOR) task or were evaluated for the Novelty effect. For the NOR task, mice performed a training session in which they were allowed to freely explore two equal objects for 10 min, and 24 hrs later (day 5) performed a 5 min retention session, where one of the familiar objects was replaced by a novel object. To evaluate the Novelty effect, mice performed a 10 min Novelty session where they were allowed to explore two equal novel objects and euthanized immediately. Control mice were placed in the arena in the absence of objects and the same procedure was performed.