Fig. 2.

Oxpecker deletion results in the de-repression of retrotransposons in the female germline. (A) RNA-seq comparison between transcriptomes of Oxp⁺/Oxp⁺and Oxp¹/Oxp¹ovaries reveal selected TEs (e.g., gypsy12, 1731, and 412 retrotransposons) that are upregulated upon Oxp deletion. However, the bulk of TEs are not affected by Oxp deletion, including the TART and TAHRE retrotransposons that are highly upregulated in HP1D homozygous mutant ovaries. (B) qPCR analyses of ovary-derived cDNA from either Oxp¹/Oxp¹flies or Oxp¹/Oxp¹flies with a “rescue” Oxp transgene confirms that TE overexpression (gypsy12, 1731, 412) in the imprecise deletion ovaries can be attributed to the loss of Oxp expression (“**”  P < 0.05).