Fig. 4.

Expression analyses of HP1D paralogs in adult tissues of two Drosophila species. All HP1D paralogs analyzed in the RT-PCR analyses of adult tissues from either D. takahashii or D. kikkawai are indicated on the left, along with the previously measured expression pattern of their D. melanogaster orthologs. The rp49 gene is shown as a loading control on the bottom and as a quality measure of the cDNA versus genomic DNA (notice size difference). For adults, heads, germline tissue (testes or ovaries), and carcass were assayed. Note that many paralogs are specific to either species. When paralogs are not present, we indicate that as “—” [n.d.* refers to not determined because no adult expression was discovered (Levine et al. 2012)].