Figure 4. Recruitment of TBK1 to Salmonella enterica serovar Typhimurium via NAP1 or NDP52, but not TANK, restricts bacterial proliferation and recruits WIPI1.

• A–D
  Analysis of Tbk1 ^−/− MEFs complemented with the indicated Flag‐tagged TBK1_ΔC‐adaptor fusion proteins. S. Typhimurium replication kinetics (A, D). Infected cells were lysed at the indicated time points post‐inoculation (p.i.), and bacteria were enumerated by their ability to form colonies on agar plates. Mean and SD of triplicate MEF cultures and duplicate colony counts. Data are representative of at least two repeats. Statistical significance to Tbk1 ^−/− MEFs expressing TBK1_ΔC is indicated. Western blot for Flag‐tagged TBK1 variants in post‐nuclear cell lysates. (B) Percentage of GFP:WIPI1‐positive S. Typhimurium at 1 h p.i. in the indicated complemented MEFs. Mean and SEM of four independent experiments. > 200 bacteria counted per coverslip. *P < 0.05, ***P < 0.001, one‐way ANOVA with Dunnett's multiple comparisons test. (C) TBK1–Nap1–NDP52 complex. N‐ and C‐termini, domains and binding partners are indicated.