Construct name	Further construct information	References
TBK1	Full‐length TBK1
TBK1ΔC	TBK1 N685, lacks the C‐terminal adaptor‐binding region
TBK1 K38M	TBK1 catalytic mutant	Pomerantz and Baltimore (1999)
TBK1ΔC K38M	TBK1 N685 K38M
TBK1ΔC:Nap1	TBK1 N685 fused to full‐length Nap1
TBK1ΔC:Nap1 ΔN85	TBK1 N685 fused to Nap1 ΔN85, Nap1 lacks the CC for binding NDP52	Ryzhakov and Randow (2007)
TBK1ΔC:Nap1 N85	TBK1 N685 fused to N85 of Nap1	Ryzhakov and Randow (2007)
TBK1ΔC:Tank	TBK1 N685 fused to full‐length Tank
TBK1ΔC:NDP52	TBK1 N685 fused to full‐length NDP52
TBK1ΔC:NDP52ΔSkich	TBK1 N685 fused to NDP52 ΔN127, NDP52 lacks the Skich domain	Gurung (2003)
TBK1ΔC:NDP52 Skich	TBK1 N685 fused to N127 of NDP52 (the Skich domain)	Gurung (2003)
TBK1ΔC:NDP52 L374A	TBK1 N685 fused to NDP52 L374A, a galectin‐8‐binding mutant of NDP52	Thurston et al (2012), Li et al (2013a)
TBK1ΔC:NDP52 D439K	TBK1 N685 fused to NDP52 D439K, a ubiquitin‐binding mutant of NDP52	This study
TBK1ΔC:NDP52 L374A+D439K	TBK1 N685 fused to NDP52 L374A + D439K, a mutant of NDP52 lacking ubiquitin binding and galectin‐8 binding	This study
TBK1ΔC:Gal1	TBK1 N685 fused to full‐length galectin‐1
TBK1ΔC:Gal8	TBK1 N685 fused to full‐length galectin‐8
TBK1ΔC:Nemo	TBK1 N685 fused to full‐length Nemo
TBK1ΔC:NemoΔZnF	TBK1 N685 fused to Nemo ΔC389, lacking the C‐terminal ZnF	Bloor et al (2008)
TBK1ΔC:NemoD304N	TBK1 N685 fused to Nemo D304N, a ubiquitin‐binding mutant of Nemo	Bloor et al (2008)
TBK1ΔC:Optn	TBK1 N685 fused to full‐length optineurin
TBK1ΔC:Optn D474N	TBK1 N685 fused to Optn D474N, a ubiquitin‐binding mutant	Zhu et al (2007)
TBK1ΔC:Optn ΔLIR	TBK1 N685 fused to Optn F178S+V179S+I181S, an LC3 interacting mutant	Wild et al (2011)
TBK1ΔC:Tab2 NZF	TBK1 N685 fused to Tab2 AA663–693	Kulathu et al (2009)
TBK1ΔC:Mud1 UBA	TBK1 N685 fused to Mud1 UBA AA293–332	Trempe et al (2005)