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. 2016 Sep 2;6:32750. doi: 10.1038/srep32750

Figure 4. Adipocyte differentiation in BCL11B−/− mouse embryonic fibroblasts (MEFs).

Figure 4

Total RNA and protein were isolated from MEFs prepared from BCL11B+/+ and BCL11B−/− embryos at the indicated times after treatment for adipocyte differentiation, as described under “Methods.” (a) BCL11B protein content of whole cell extracts assessed using SDS−PAGE and immunoblotting (IB) with anti-BCL11B or anti-β-actin antibodies. Shown here are representative results of at least three replicate experiments. (b) Relative mRNA levels of the adipocyte marker genes C/EBPα, PPARγ2, perilipin, and aP2, assessed using real-time PCR with normalization to 18S rRNA. The mRNA levels of BCL11B+/+ MEFs that had not been treated for adipocyte differentiation are represented as 1. All data are expressed as means ± S.E. (n = 3). *P < 0.05; **P < 0.01. (c) Control and knockdown cells stained with Oil Red O at 192 h (day 8) after differentiation.