Table 2.
Immunohistochemical detection of brachyury in biopsies obtained from primary lesions of TNBC patients.
Nuclear brachyury | Cytoplasmic brachyury | Total brachyury | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Pt | Stage | Ki67 | p53 | Pos (%) | SI | Index | Pos (%) | SI | Index | Pos (%) | SI | Index |
1 | I | 90 | NEG | 100 | +++ | 300 | 70 | ++ | 140 | 100 | +++ | 300 |
2 | I | 25 | POS | 100 | +++ | 300 | 100 | ++ | 200 | 100 | +++ | 300 |
3 | I | 35 | POS | 70 | +++ | 210 | 100 | ++ | 200 | 100 | +++ | 300 |
4 | I | 60 | POS | 60 | +++ | 180 | 80 | ++ | 160 | 90 | +++ | 270 |
5 | I | 80 | NEG | 90 | +++ | 270 | 10 | + | 10 | 90 | +++ | 270 |
6 | I | 60 | NEG | 20 | ++ | 40 | 80 | +++ | 240 | 80 | +++ | 240 |
7 | I | 80 | NEG | 80 | ++ | 160 | 50 | + | 50 | 80 | ++ | 160 |
8 | I | 90 | POS | 20 | + | 20 | 80 | + | 80 | 80 | + | 80 |
9 | I | 90 | POS | 30 | + | 30 | 80 | ++ | 160 | 80 | ++ | 160 |
10 | I | 40 | NEG | 70 | ++ | 140 | 10 | + | 10 | 70 | ++ | 140 |
11 | I | 70 | POS | 50 | +++ | 150 | 70 | + | 70 | 70 | +++ | 210 |
12 | I | 20 | POS | 40 | +++ | 120 | 60 | ++ | 120 | 70 | +++ | 210 |
13 | I | 70 | NEG | 20 | + | 20 | 70 | ++ | 140 | 70 | ++ | 140 |
14 | I | 20 | NEG | 70 | +++ | 210 | 70 | + | 70 | 70 | +++ | 210 |
15 | I | 35 | POS | 60 | +++ | 180 | 20 | + | 20 | 60 | +++ | 180 |
16 | I | 40 | POS | 60 | ++ | 120 | 40 | + | 40 | 60 | ++ | 120 |
17 | I | 60 | POS | 20 | + | 20 | 50 | + | 50 | 50 | + | 50 |
18 | I | 80 | NEG | 20 | ++ | 40 | 50 | + | 50 | 50 | ++ | 100 |
19 | I | 90 | POS | 40 | ++ | 80 | 40 | + | 40 | 40 | ++ | 80 |
20 | I | 50 | NEG | 40 | ++ | 80 | 10 | + | 10 | 40 | ++ | 80 |
21 | I | 25 | NEG | 10 | + | 10 | 40 | + | 40 | 40 | + | 40 |
22 | I | 40 | NEG | 25 | ++ | 50 | 10 | + | 10 | 30 | + | 60 |
23 | I | 40 | NEG | 15 | ++ | 30 | 20 | + | 20 | 25 | + | 50 |
24 | I | 70 | POS | 15 | + | 15 | 15 | + | 15 | 20 | + | 20 |
25 | I | 80 | POS | 20 | + | 20 | 10 | + | 10 | 20 | + | 20 |
26 | I | 40 | POS | 20 | ++ | 40 | 0 | - | 0 | 20 | ++ | 40 |
27 | I | 70 | NEG | 10 | ++ | 20 | 10 | + | 10 | 15 | ++ | 30 |
28 | I | 90 | POS | 0 | - | 0 | 0 | - | 0 | 0 | - | 0 |
29 | IIA | 25 | POS | 100 | ++ | 200 | 10 | + | 10 | 100 | ++ | 200 |
30 | IIA | 90 | POS | 80 | +++ | 240 | 80 | ++ | 160 | 100 | +++ | 300 |
31 | IIA | 60 | POS | 20 | +++ | 60 | 100 | ++ | 200 | 100 | +++ | 300 |
32 | IIA | 90 | POS | 80 | +++ | 240 | 100 | +++ | 300 | 100 | +++ | 300 |
33 | IIA | 40 | POS | 100 | +++ | 300 | 10 | ++ | 20 | 100 | +++ | 300 |
34 | IIA | 45 | POS | 30 | + | 30 | 100 | ++ | 200 | 100 | ++ | 200 |
35 | IIA | 85 | POS | 90 | +++ | 270 | 20 | + | 20 | 90 | +++ | 270 |
36 | IIA | 90 | POS | 80 | +++ | 240 | 60 | + | 60 | 90 | +++ | 270 |
37 | IIA | 90 | NEG | 70 | +++ | 210 | 80 | + | 80 | 80 | +++ | 240 |
38 | IIA | 25 | NEG | 80 | +++ | 240 | 10 | + | 10 | 80 | +++ | 240 |
39 | IIA | 70 | POS | 30 | ++ | 60 | 80 | + | 80 | 80 | ++ | 160 |
40 | IIA | 40 | POS | 80 | ++ | 160 | 50 | ++ | 100 | 80 | ++ | 160 |
41 | IIA | 90 | NEG | 70 | +++ | 210 | 20 | ++ | 40 | 70 | +++ | 210 |
42 | IIA | 35 | POS | 50 | ++ | 100 | 30 | + | 30 | 50 | ++ | 100 |
43 | IIA | 60 | NEG | 25 | ++ | 50 | 10 | + | 10 | 30 | ++ | 60 |
44 | IIA | 70 | POS | 10 | + | 10 | 0 | - | 0 | 10 | + | 10 |
45 | IIA | 60 | POS | 10 | + | 10 | 0 | - | 0 | 10 | + | 10 |
46 | IIA | 90 | NEG | 10 | + | 10 | 0 | - | 0 | 10 | + | 10 |
47 | IIA | 25 | NEG | 5 | + | 5 | 0 | - | 0 | 5 | + | 5 |
48 | IIB | 35 | POS | 75 | ++ | 150 | 100 | + | 100 | 100 | ++ | 200 |
49 | IIB | 20 | POS | 20 | ++ | 40 | 80 | + | 80 | 80 | + | 160 |
50 | IIB | 60 | NEG | 40 | +++ | 120 | 70 | ++ | 140 | 70 | +++ | 210 |
51 | IIB | 80 | NEG | 70 | +++ | 210 | 30 | + | 30 | 70 | +++ | 210 |
52 | IIB | 90 | NEG | 50 | ++ | 100 | 50 | + | 50 | 60 | ++ | 120 |
53 | IIB | 80 | NEG | 60 | ++ | 120 | 20 | + | 20 | 60 | ++ | 120 |
54 | IIB | 90 | POS | 30 | +++ | 90 | 20 | ++ | 40 | 40 | +++ | 120 |
55 | IIB | 90 | NEG | 30 | + | 30 | 30 | + | 30 | 30 | + | 30 |
56 | IIIA | 90 | POS | 80 | +++ | 240 | 100 | + | 100 | 100 | +++ | 300 |
57 | IIIA | 90 | NEG | 100 | +++ | 300 | 0 | - | 0 | 100 | +++ | 300 |
58 | IIIA | 10 | NEG | 90 | +++ | 270 | 50 | ++ | 100 | 90 | +++ | 270 |
59 | IIIA | 30 | NEG | 90 | + | 90 | 30 | + | 30 | 90 | + | 90 |
60 | IIIA | 60 | NEG | 80 | ++ | 160 | 60 | ++ | 120 | 90 | ++ | 180 |
61 | IIIA | 80 | NEG | 70 | +++ | 210 | 60 | + | 60 | 80 | +++ | 240 |
62 | IIIA | 70 | NEG | 70 | +++ | 210 | 10 | + | 10 | 70 | +++ | 210 |
63 | IIIA | 70 | NEG | 40 | + | 40 | 60 | + | 60 | 60 | + | 60 |
64 | IIIA | 50 | NEG | 50 | ++ | 100 | 10 | + | 10 | 50 | ++ | 100 |
65 | IIIA | 60 | NEG | 40 | + | 40 | 15 | + | 15 | 45 | + | 45 |
66 | IIIB | 95 | POS | 40 | +++ | 120 | 100 | + | 100 | 100 | ++ | 200 |
67 | IIIB | 35 | NEG | 100 | +++ | 300 | 50 | ++ | 100 | 100 | +++ | 300 |
68 | IIIB | 70 | NEG | 100 | +++ | 300 | 30 | + | 30 | 100 | ++ | 200 |
69 | IIIB | 80 | NEG | 100 | +++ | 300 | 10 | + | 10 | 100 | +++ | 300 |
70 | IIIB | 80 | NEG | 90 | +++ | 270 | 20 | + | 20 | 90 | +++ | 270 |
71 | IIIB | 40 | POS | 80 | +++ | 240 | 40 | + | 40 | 80 | +++ | 240 |
72 | IIIB | 50 | POS | 60 | ++ | 120 | 60 | ++ | 120 | 80 | ++ | 160 |
73 | IIIB | 80 | NEG | 70 | ++ | 210 | 70 | + | 70 | 80 | +++ | 240 |
74 | IIIB | 90 | NEG | 50 | +++ | 150 | 50 | + | 50 | 50 | +++ | 150 |
75 | IIIB | 90 | POS | 30 | + | 30 | 30 | + | 30 | 40 | + | 40 |
76 | IV | 90 | NEG | 30 | ++ | 60 | 40 | + | 40 | 50 | ++ | 100 |
77 | IV | 80 | NEG | 15 | +++ | 45 | 10 | + | 10 | 10 | +++ | 30 |
SI: Staining Intensity; NEG: negative; POS: positive.