Figure 3.

The effect of sectors on transposition rate is not a by-product of positional conservation. Sector and conserved residues were split into three groups: sector residues with low positional conservation (D < 0.5), sector residues with high positional conservation, and conserved residues that are not part of any sector. Transposition rates of mutants (median, box: 25–75%, whiskers: 10–90%) in all three groups are significantly different from mutants in other residues (P << 0.05 for all comparisons, Fisher post-hoc tests, analysis of variance on log transformed transposition rates), and there is no significant difference between the mutants of nonconserved sector and conserved but nonsector residues (P > 0.05 in all three alignments, Fisher post-hoc tests).