Figure 6.

The effect of DNA-protein and protein-protein interactions on transposition rate. When outliers are excluded, mutants of residues interacting with DNA (“DNA”) and the other SB chain (“PPI”) have significantly lower transposition rates (P < 0.05, Mann-Whitney U-test) than other residues located at the surface (RSA > 0.1). Surprisingly, 12 of the 14 hyperactive mutants (outliers, 300+% activity) are also located in the protein surface, and none in the DNA binding regions, suggesting that the modification of protein-protein interactions might be responsible for their dramatically increased activity. (Outliers with identical transposition rates were shifted by 10%, for visibility)