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. 2016 Aug 29;9:5349–5360. doi: 10.2147/OTT.S107957

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© 2016 Jiang et al. This work is published and licensed by Dove Medical Press Limited

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ALDH1A1 regulates the JAK2/STAT3 pathway in Pfeiffer cells.

Notes: (A) Effects of ALDH1A1 on the expressions of JAK2 and p-JAK2 (Y570, Y931, and Y1007) were analyzed by Western blotting. (B) Expression levels of STAT3 and p-STAT3 (Y705, S727) were analyzed by Western blotting. GAPDH blotting was used as a loading control. Bar graphs are derived from densitometric scanning of the blots. The density of the ALDH1A1, JAK2/p-JAK2, and STAT3/p-STAT3 blots was normalized against that of GAPDH to obtain a relative blot density. Bars are mean ± SD from three independent experiments. *P<0.05.

Abbreviations: ALDH1A1, aldehyde dehydrogenase 1A1; SD, standard deviation; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; shRNA, short hairpin RNA; Pfeiffer-NC, cells transfected with empty vector; Pfeiffer-shRNA, ALDH1A1-shRNA vector; Pfeiffer-ALDH1A1, ALDH1A1 expression vector; p-STAT3, phosphorylated-STAT3; p-JAK2, phosphorylated-JAK2.