Figure 2.
Effect of DcR3-specific siRNA on cell apoptosis in glioma cells.
Notes: U251MG, LN-308, and U87MG cells (2.5×103 cells per well in 96-well plate) were cultured for 24 hours and then transfected with negative siRNA, scrambled siRNA, and DcR3-specific siRNA and incubated for up to another 96 hours. Apoptosis (U251MG (A), LN-308 (B), and U87MG (C)) and caspase-3/7 activity (U251MG (D), LN-308 (E), and U87MG (F)) were assessed by the Hoechst 33342/PI double-staining assay and fluorescent caspase-3/7 assay, respectively. *P<0.05, **P<0.01, and ***P<0.001 compared to negative siRNA controls and scrambled siRNAs at the same time point.
Abbreviations: siRNA, small interfering RNA; PI, propidium iodide.