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. 2016 Aug 29;9:5195–5202. doi: 10.2147/OTT.S108395

Table 2.

Cell growth inhibitory effect of DcR3-specific siRNA using different techniques and cell lines

Time (hours) U251MG
LN-308
U87MG
Scrambled siRNA (%) DcR3 siRNA (%) Scrambled siRNA (%) DcR3 siRNA (%) Scrambled siRNA (%) DcR3 siRNA (%)
MTS tetrazolium assay 0 100.333±1.528 101.333±1.528 101.000±2.646 102.000±1.000 99.667±0.577 100.667±1.528
24 100.667±1.154 98.667±2.517 101.000±1.732 99.667±3.215 99.667±0.577 98.000±2.646
48 100.000±1.000 91.333±2.082 100.333±1.155 95.000±4.359 101.333±0.577 92.667±0.577
72 100.667±2.082 76.333±5.131 101.667±2.309 80.667±4.042 101.000±2.000 76.333±5.132
96 101.667±0.577 64.333±5.859 100.667±1.528 72.667±3.512 100.667±1.528 68.333±4.042
Fluorimetric resorufin viability assay 0 101.333±0.577 101.000±1.000 101.667±1.155 100.000±1.000 101.333±1.528 99.333±0.577
24 101.667±2.517 97.667±4.041 102.000±2.000 99.000±4.359 101.000±1.000 100.667±2.517
48 100.000±3.000 89.333±3.512 99.000±1.732 87.333±4.163 100.000±3.000 93.333±1.528
72 100.333±1.528 80.667±2.309 99.000±1.000 70.333±5.686 100.333±2.517 86.000±5.000
96 102.333±2.517 62.333±2.082 101.000±1.000 63.667±9.292 100.667±1.528 80.000±3.606

Abbreviation: siRNA, small interfering RNA.