Table 2.
Time (hours) | U251MG
|
LN-308
|
U87MG
|
||||
---|---|---|---|---|---|---|---|
Scrambled siRNA (%) | DcR3 siRNA (%) | Scrambled siRNA (%) | DcR3 siRNA (%) | Scrambled siRNA (%) | DcR3 siRNA (%) | ||
MTS tetrazolium assay | 0 | 100.333±1.528 | 101.333±1.528 | 101.000±2.646 | 102.000±1.000 | 99.667±0.577 | 100.667±1.528 |
24 | 100.667±1.154 | 98.667±2.517 | 101.000±1.732 | 99.667±3.215 | 99.667±0.577 | 98.000±2.646 | |
48 | 100.000±1.000 | 91.333±2.082 | 100.333±1.155 | 95.000±4.359 | 101.333±0.577 | 92.667±0.577 | |
72 | 100.667±2.082 | 76.333±5.131 | 101.667±2.309 | 80.667±4.042 | 101.000±2.000 | 76.333±5.132 | |
96 | 101.667±0.577 | 64.333±5.859 | 100.667±1.528 | 72.667±3.512 | 100.667±1.528 | 68.333±4.042 | |
Fluorimetric resorufin viability assay | 0 | 101.333±0.577 | 101.000±1.000 | 101.667±1.155 | 100.000±1.000 | 101.333±1.528 | 99.333±0.577 |
24 | 101.667±2.517 | 97.667±4.041 | 102.000±2.000 | 99.000±4.359 | 101.000±1.000 | 100.667±2.517 | |
48 | 100.000±3.000 | 89.333±3.512 | 99.000±1.732 | 87.333±4.163 | 100.000±3.000 | 93.333±1.528 | |
72 | 100.333±1.528 | 80.667±2.309 | 99.000±1.000 | 70.333±5.686 | 100.333±2.517 | 86.000±5.000 | |
96 | 102.333±2.517 | 62.333±2.082 | 101.000±1.000 | 63.667±9.292 | 100.667±1.528 | 80.000±3.606 |
Abbreviation: siRNA, small interfering RNA.