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A, Cells were treated for 24 hrs with PHTPP (100nm), E2 + PHTPP or DPN + PHTPP. (n = 5). B, Western blot showing relative protein levels. Polyclonal anti-COX-2 (Abcam) and anti-β-actin (Cell signaling) were used at a dilution of 1:1000. Data represents mean ± SEM. A, (*) p = 0.001 E2 and DPN compared to VEH, (#) p = 0.023 E2+PHTPP compared to E2, (^) p = 0.026 DPN+PHTPP compared to DPN.
