Fig 3. E2 and DPN fail to induce ERβ occupancy at the cox-2 proximal promoter in the region of the NF-κB element.

A, Schematic of cox-2 proximal promoter region. The numbers indicate the bases from the transcription start site (arrow). (P1) forward primer, (P2) reverse primer. B and C, Cells were treated for 24 hrs here and in subsequent experiments. Fold occupancy at the cox-2 NF-κB and c-fos ERE regions, respectively. A polyclonal anti-ERβ antibody (Abcam) was used. (n = 5). Data represent mean ± SEM C, (*) p = 0.043, E2 compared to VEH, and p = 0.008, DPN compared to VEH.