Table 3. Effect of a single CPP-ACP treatment on an established 16 h S. mutans biofilm cultured in a 3-channel flow cell system as determined using COMSTAT analysis of CLSM images.

Values are the mean and standard deviation of three biological replicates. The percentage change relative to the control is shown in brackets.

	Control (no CPP-ACP)	0.5% CPP	1% CPP-ACP
Biovolume (μm3/μm2)	4.22 ± 1.18	3.61 ± 1.11 (-14.4%)	1.76 ± 0.68* (-58.4%)
Average thickness (μm)	7.63 ± 2.29	6.10 ± 1.92 (-20.1%)	3.23 ± 1.53* (-57.7%)
Maximum thickness (μm)	28.4 ± 4.08	28.67 ± 4.32 (+0.9%)	24.4 ± 3.94 (-14.08%)
Surface area: Volume ratio	1.98 ± 0.38	2.07 ± 0.47 (+4.2%)	2.45 ± 0.34 (+19.2%)
Proportion of the substratum colonised (%)	13.19 ± 6.52	15.23 ± 5.09 (+13.4%)	9.89 ± 2.90 (-25%)

* Significantly different from control p < 0.05