Fig. 3.

In vitro cellular uptake analysis of sigma receptor competition, existence of sigma receptors in OSCC cells and effect of anisamide targeting of LCP on sub-cellular distribution of delivered dsDNA cargo in cultured SCC4 cells. (A) Sigma receptor binding competition analysis of untreated, free DNA, DNA in non-targeted (no anisamide) LCP NP and DNA in targeted (with anisamide) LCP NP using haloperidol, the sigma receptor antagonist. (B) Western blots on examination of sigma receptor of SCC4 and SAS cell lines. (C, D) Texas Red-labeled HIF1α dsDNA was used as a model for siRNA and delivered to SCC4 cells using either AA-targeted or untargeted control LCP-PEG in order to investigate the effects of nanoparticle targeting on subcellular distribution of delivered cargo. Cells were visualized by confocal microscopy 40 minutes after LCP treatment. Nuclei were stained with DAPI (blue) and cell membranes were stained with wheat germ agglutinin (green). Arrows indicate the location of HIF1α dsDNA (red). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)