Table 1. Sequencing data overview of three camel samples.
Sample ID | Raw data | Clean Data (%) | Merged (%) | V-align (%) | J-align (%) | VJ-align (%) | Effective data | Effective data/raw data (%) |
---|---|---|---|---|---|---|---|---|
1-VH | 585688 | 74.3 | 92.09 | 71.31 | 91.66 | 70.46 | 351209 | 59.97 |
1-VHH | 610474 | 66.74 | 91.71 | 53.27 | 90.82 | 52.54 | 309782 | 50.74 |
2-VH | 461284 | 73.69 | 92.47 | 70.18 | 91.72 | 69.29 | 272373 | 59.05 |
2-VHH | 540738 | 64.22 | 91.71 | 53.67 | 91.21 | 52.82 | 266320 | 49.25 |
3-VH | 544848 | 75.67 | 91.95 | 58.02 | 88.69 | 57.33 | 310279 | 56.95 |
3-VHH | 573617 | 71.64 | 91.12 | 45.52 | 90.02 | 44.84 | 292751 | 51.04 |
Sample ID: “1-VH” and “1-VHH” represent the NO.1 camel`s conventional antibody and nanobody clones, respectively. The same nomenclature is used in the other samples. Raw data are the total number of reads; Clean data (%) is the rate of the filtered sequence reads, with the low-quality data removed; Merged (%) is the percent of sequences generated by merging the pair-end reads to one intact read; V-align (%), J-align (%) and VJ-align (%) are percentages of the sequencing data that were matched with the IMGT reference sequences; Effective data are the number of reads after filtering; Effective data/raw data (%) is the percent of raw data that remained after filtering.