Table 1. Effect of different chromosomal positions for datA, DARS1, DARS2 in MG1655.

Strains	RD to oriCa	DTb	Origin/massc	Asynchrony indexd
Wild-type		40 min	1	0.05
ΔdatA	0.10	41 min	1.25 (0.05)	0.40
ΔDARS1	0.33	40 min	0.82 (0.01)	0.06
ΔDARS2	0.21	41 min	0.82 (0.01)	0.30
ΔdatA CAG18499::datA::cat	0.01	36 min	0.98 (0.01)	0.06
ΔdatA CAG18496::datA::cat	0.02	37 min	0.97 (0.02)	0.06
ΔdatA CAG18488::datA::cat	0.09	40 min	0.97 (0.04)	0.06
ΔdatA CAG12135::datA::cat	0.21	ND	ND	ND
ΔdatA CAG12173::datA::cat	0.22	39 min	1.01	0.06
ΔdatA CAG18493::datA::cat	0.33	38 min	1.06 (0.01)	0.05
ΔdatA CAG18461::datA::cat	0.48	40 min	1.12 (0.02)	0.06
ΔdatA CAG12151::datA::cat	0.46	38 min	1.09 (0.01)	0.06
ΔdatA CAG12151::datA::cat CAG18461::datA		43 min	0.95 (0.04)	0.08
ΔDARS1 CAG18499::DARS1::cat	0.01	41 min	1.10 (0.04)	0.09
ΔDARS1 CAG18496::DARS1::cat	0.02	38 min	1.09 (0.03)	0.08
ΔDARS1 CAG18488::DARS1::cat	0.09	40 min	1.03 (0.01)	0.06
ΔDARS1 CAG12135::DARS1::cat	0.21	40 min	1.01 (0.04)	0.09
ΔDARS1 CAG12173::DARS1::cat	0.22	40 min	0.99	0.09
ΔDARS1 CAG18493::DARS1::cat	0.33	43 min	0.96 (0.02)	0.08
ΔDARS1 CAG18461::DARS1::cat	0.48	40 min	0.97 (0.02)	0.06
ΔDARS1 CAG12151::DARS1::cat	0.46	42 min	0.97 (0.00)	0.08
ΔDARS2 CAG18499::DARS2::cat	0.01	41 min	0.99 (0.04)	0.10
ΔDARS2 CAG18496::DARS2::cat	0.02	40 min	1.02 (0.04)	0.10
ΔDARS2 CAG18488::DARS2::cat	0.09	40 min	0.99 (0.01)	0.10
ΔDARS2 CAG12135::DARS2::cat	0.21	42 min	1.01 (0.02)	0.08
ΔDARS2 yafJ::DARS2::cat	0.21	40 min	1.00	0.11
ΔDARS2 CAG12173::DARS2::cat	0.22	ND	ND	ND
ΔDARS2 CAG18493::DARS2::cat	0.33	42 min	0.98 (0.01)	0.10
ΔDARS2 CAG18461::DARS2::cat	0.48	42 min	0.95 (0.05)	0.27
ΔDARS2 CAG12151::DARS2::cat	0.46	42 min	0.96 (0.04)	0.41

^a Relative distance from oriC to insertion (see Materials and Methods).

^b Doubling time in minimal medium supplemented with glucose and casamino acids grown at 37°C

^c Determined as average light scatter from flow cytometric analysis. Numbers are normalized to 1 for wild-type. Standard error is indicated in the brackets.

^d Asynchrony index; calculated as described in Materials and Methods.