Table II.
Allergen-specific scFv isolated from combinatorial libraries based on IGHV with origin in the IgE repertoire.
| Donor | Clone ID | Allergen | IGHV | IGHV mutations (%) | CDRH3 | Identified by HTS |
|---|---|---|---|---|---|---|
| 1 | IT1-B215 | Bet v 2 | 1–18 | 0 | ARVRSSGYGNWFDP | |
| 1 | IT1-B228 | Bet v 2 | 1–69 | 0 | ARDNGGEGY | |
| 1 | IT1-B237 | Bet v 2 | 3–21 | 4.2 | ARGGTRYFAS | |
| 1 | IT1-B248 | Bet v 2 | 3–11 | 0 | ARDRSPIAAARLAFDI | |
| 2 | IT2-B12 | Bet v 1 | 5–51 | 9.8 | ARLGGGSRGWYYYYGMDV | Yes |
| 2 | IT2-B15 | Bet v 1 | 3–48 | 5.3 | ARGYYDGRKLRN | |
| 2 | IT2-B116 | Bet v 1 | 3–11 | 2.6 | ARDAYFDWSLDY | Yes |
| 2 | IT2-B23 | Bet v 2 | 3–66 | 5.0 | ARGYYDGRKLRN | Yes |
| 2 | IT2-B227 | Bet v 2 | 3–74 | 3.8 | ARRDVAVVPGATGDNYYYGLDV | Yes |
| 2 | IT2-B239 | Bet v 2 | 1–18 | 3.0 | ARSLFLLSPKARGYYYYGMDV | Yes |
| 2 | IT2-P11 | Phl p 1 | 3–23 | 4.5 | ANLGFDY | Yes |
| 2 | IT2-P514 | Phl p 5 | 1–69 | 8.7 | ARALIPLESQAAD | Yes |
| 2 | IT2-P536 | Phl p 5 | 1–69 | 5.7 | ASMGRGYCSGDNCYNFDH | Yes |
| 2 | IT2-P64 | Phl p 6 | 5–51 | 3.8 | ARRFGGDWFGSLGWYYFDH | Yes |
| 2 | IT2-P614 | Phl p 6 | 3–48 | 3.4 | ARDRGTPWHYDGMDV | Yes |
| 3 | IT3-B11 | Bet v 1 | 1–46 | 10.2 | ASDIAEGLGQHLFDH | |
| 3 | IT3-B116 | Bet v 1 | 3–30 | 6.8 | ASSLTSTGMGRY | |
| 3 | IT3-B229 | Bet v 2 | 1–69 | 12.1 | ARDRKFYYDRSGVPYFDH | |
| 3 | IT3-P11 | Phl p 1 | 4-4 | 3.4 | ARDGKNGSSDY | Yes |
| 4 | IT4-B119 | Bet v 1 | 3–48 | 4.2 | ARGPGYSSSWYGYYFDS | Yes |
| 4 | IT4-B148 | Bet v 1 | 3–48 | 11.3 | ASPPTSYDFWSDYSDYDYYYMDV | Yes |
| 4 | IT4-B225 | Bet v 2 | 3–30 | 3.4 | ARDLTGNFAN | Yes |
| 4 | IT4-B229 | Bet v 2 | 3–20 | 9.8 | ARDLIGNFAN | |
| 4 | IT4-P517 | Phl p 5 | 3–7 | 4.5 | ARDSRQWWFHIEGDAFDI | Yes |
| 4 | IT4-P62 | Phl p 6 | 3–33 | 7.6 | ARDFFPLAVLSPPLGY | Yes |
| 5 | IT5-nD134 | Der p 1 | 3–53 | 0.8 | AREGGVAARPNPDAFDI | Yes |
| 5 | IT5-rD13 | Der p 1 | 3–11 | 5.3 | ARDGALVWFGNKSYGMDV | Yes |
| 5 | IT5-rD120 | Der p 1 | 3–23 | 5.7 | AKAHGFGSPGWGSGWHRKTPSRYPYYFDY | Yes |
| 6 | IT6-B15 | Bet v 1 | 3–30 | 11.3 | ARGRPRAYYYDDSGSKQYWDEHYFDY | Yes |
| 6 | IT6-B22 | Bet v 2 | 3–30 | 10.6 | ARDIFASQGAADY | Yes |
| 6 | IT6-nD12 | Der p 1 | 3–53 | 8.4 | ARLYYDFWSGHAYFFYYMDV | Yes |
| 6 | IT6-nD13 | Der p 1 | 3–23 | 8.7 | AKSERPIVATITGYYYYYMDV | Yes |
| 6 | IT6-nD16 | Der p 1 | 3–30 | 9.1 | AKAAYSYGMKNSLDF | Yes |
| 6 | IT6-nD17 | Der p 1 | 3–30 | 17.0 | TRDRSPVSGVLQH | Yes |
| 6 | IT6-rD111 | Der p 1 | 3–64 | 6.0 | VKNMVRGVITDAFEI | Yes |
| 6 | IT6-rD116 | Der p 1 | 3–49 | 14.4 | SRGLWFGKLWGPPREH | Yes |
| 6 | IT6-rD128 | Der p 1 | 3–30 | 10.9 | ARDEGGDSSGNH | Yes |
| 7 | IT7-B14 | Bet v 1 | 1–2 | 13.6 | ARGLRSQLWYLDV | Yes |
| 7 | IT7-B22 | Bet v 2 | 3–23 | 0.4 | APSRYCSGGSCYSGY | |
| 7 | IT7-B210 | Bet v 2 | 3–23 | 9.1 | VKEKGWQQLPKGGHNWFDP | Yes |
| 7 | IT7-B214 | Bet v 2 | 3–7 | 6.0 | TRQSGWLPFKD | Yes |
| 7 | IT7-B215 | Bet v 2 | 3–21 | 6.0 | ARDLSYSGGD | |
| 7 | IT7-B222 | Bet v 2 | 3–30 | 0 | AKAPRSSSFRYFQH | |
| 7 | IT7-P51 | Phl p 5 | 3–21 | 2.6 | ARERSPWSEEAFDV | Yes |
| 7 | IT7-P517 | Phl p 5 | 3–23 | 9.8 | AKDGISEYCSGGSCHSRGWVYFDY | Yes |
| 7 | IT7-P61 | Phl p 6 | 3–21 | 1.5 | TRSPYYSGSGSYLDN | Yes |
| 8 | IT8-B13 | Bet v 1 | 3–49 | 4.8 | TRRATLYYDTSGYSYYFDY | Yes |
| 8 | IT8-B22 | Bet v 2 | 1–18 | 0 | ARGVGSRRQVYYYGMDV | Yes |
| 8 | IT8-B216 | Bet v 2 | 3–11 | 0 | ARVGRVRGVIRFDP | |
| 8 | IT8-B243 | Bet v 2 | 3–23 | 2.3 | AKGGSGTISTP | |
| 8 | IT8-P220 | Phl p 2 | 4–31 | 7.1 | ARGAGDFDS | Yes |
| 8 | IT8-P621 | Phl p 6 | 3–30 | 9.4 | ARGVGDYVWGPKGDY |