Pathways for the synthesis and secretion of diacylglycerol (DG) in fat body. Fatty acids (FA) entering the fat body, produced de novo, or released from triacylglycerol (TG) by the action of lipases (TGL, ATGL and HSL) on the lipid droplets are reused in part to form DG for export. The acyl-CoA formed by ACS could enter the synthesis of DG through the PA-pathway or through the MG-pathway, which would use monoacylglycerol (MG) produced by the hydrolysis of stored TG. Export of DG to circulating lipophorin is expected to involve the lipid transfer protein (LTP) and other membrane proteins, such as the lipophorin receptor. A similar scheme could be envisaged for the synthesis and export of DG from midgut tissue.